Fig. 5. Effect of ZNF281 on CRC cell migration and invasion. (A) Wound healing assays were performed to investigate the role of ZNF281 in SW480 and HCT116 cell migration. Left: observations by microscopy were recorded at 0 and 36 h after the cell surface was scratched. Original magnification ×100. The right panel shows the quantitative analysis of migration length of the vector and sh-ZNF281-treated cells presented as the means ± SD. *P<0.05 and **P<0.01. (B) Transwell assays were performed to investigate the role of ZNF281 in cell invasion. The images were obtained 48 h after seeding (magnification ×200). The number of cells was counted in five representative high-power fields per Transwell (left). Quantitative analysis of the number of migrating cells is presented in the right panel. Error bars represent the mean ± SD of 3 different fields. The differences between independent experimental groups were tested using an independent-samples t-test. *P<0.05 and **P<0.01.